University of Illinois at Urbana-Champaign Block I

Women's Health and Metabolism Lab

Striving to improve lives of women and breast cancer survivors using cutting edge science guided by patient perspective

Main menu

Skip to primary content
Skip to secondary content
  • Home
  • People
    • Director
    • Research Assistants
    • Graduate Students
    • Undergraduate Students
    • Alumni
  • Research
    • Women’s Health, Hormones and Nutrition
    • Role of Systemic and Cellular Metabolism in Metastasis and Therapy Resistance
    • Understanding Molecular Basis of Health Disparities
    • Data and protocols
  • Publications
  • Classes
    • FSHN 480- Basic Toxicology
    • DNS561/FSHN520 Nutrition and Women’s Health
    • FSHN 595- Diet Nutrition and Cancer
    • FSHN 295-Undergraduate Research
  • Outreach & Advocacy
  • Contact Us

Monthly Archives: September 2011

Toll-like receptor 2 (TLR2) and TLR9 signaling results in HIV-long terminal repeat trans-activation and HIV replication in HIV-1 transgenic mouse spleen cells: implications of simultaneous activation of TLRs on HIV replication, Equils O, Schito ML, Karahashi H, Madak Z, Yarali A, Michelsen KS, Sher A, Arditi M.J, Immunology. 2003 May 15; 170(10):5159-64, PMID:12734363

Posted on September 29, 2011 by zmadake2@illinois.edu

Opportunistic infections are common in HIV-infected patients; they activate HIV replication and contribute to disease progression. In the present study we examined the role of Toll-like receptor 2 (TLR2) and TLR9 in HIV-long terminal repeat (HIV-LTR) trans-activation and assessed whether TLR4 synergized with TLR2 or TLR9 to induce HIV replication. Soluble Mycobacterium tuberculosis factor (STF) and phenol-soluble modulin from Staphylococcus epidermidis induced HIV-LTR trans-activation in human microvessel endothelial cells cotransfected with TLR2 cDNA. Stimulation of ex vivo spleen cells from HIV-1 transgenic mice with TLR4, TLR2, and TLR9 ligands (LPS, STF, and CpG DNA, respectively) induced p24 Ag production in a dose-dependent manner. Costimulation of HIV-1 transgenic mice spleen cells with LPS and STF or CpG DNA induced TNF-alpha and IFN-gamma production in a synergistic manner and p24 production in an additive fashion. In the THP-1 human monocytic cell line stably expressing the HIV-LTR-luciferase construct, LPS and STF also induced HIV-LTR trans-activation in an additive manner. This is the first time that TLR2 and TLR9 and costimulation of TLRs have been shown to induce HIV replication. Together these results underscore the importance of TLRs in bacterial Ag- and CpG DNA-induced HIV-LTR trans-activation and HIV replication. These observations may be important in understanding the role of the innate immune system and the molecular mechanisms involved in the increased HIV replication and HIV disease progression associated with multiple opportunistic infections.

Posted in Toll Like Receptors

Archives

  • June 2018
  • January 2018
  • September 2016
  • August 2016
  • July 2016
  • May 2016
  • March 2016
  • December 2015
  • November 2015
  • September 2015
  • August 2015
  • May 2015
  • April 2015
  • January 2015
  • December 2014
  • November 2014
  • September 2014
  • August 2014
  • May 2014
  • November 2013
  • October 2013
  • September 2013
  • August 2013
  • July 2013
  • June 2013
  • May 2013
  • April 2013
  • March 2013
  • February 2013
  • January 2013
  • September 2012
  • September 2011

Meta

  • Log in

Meta

  • Log in
  • Entries feed
  • Comments feed
  • WordPress.org
Privacy
© 2023 University of Illinois Board of Trustees